Coding

Part:BBa_K1202011:Design

Designed by: Furkan Bestepe   Group: iGEM13_ATOMS-Turkiye   (2013-10-04)


LsrK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 61
    Illegal AgeI site found at 415
    Illegal AgeI site found at 577
    Illegal AgeI site found at 670
    Illegal AgeI site found at 855
    Illegal AgeI site found at 940
    Illegal AgeI site found at 1429
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because of the length of this part, we could not combine the LsrR and Lsr promoter and this part, though we planned this. Eventually, we ligated these two parts manually.


Source

It is originated from E.coli. GeneScript synthesized the sequence we found through our researches.

References